Semi-automated quantification of methylmalonic acid in human serum by LC-MS/MS
نویسندگان
چکیده
منابع مشابه
Semi-automated quantification of methylmalonic acid in human serum by LC-MS/MS.
BACKGROUND Methylmalonic acid (MMA), a sensitive biomarker of functional vitamin B12 deficiency, is commonly determined by gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods using manual extraction and derivatization of MMA to reduce polarity prior to separation. METHODS In the present study we introduce a semi-automated extractio...
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Determination of methylmalonic acid (MMA) in serum has been established as an accurate test for the diagnosis of cobalamin deficiency. We describe here the development and performance of a liquid-chromatographic assay of MMA in blood and urine. The assay is based on our recent finding that one of the carboxylic acid moieties of some short-chain dicarboxylic acids reacts with the fluorogenic rea...
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ژورنال
عنوان ژورنال: Scandinavian Journal of Clinical and Laboratory Investigation
سال: 2012
ISSN: 0036-5513,1502-7686
DOI: 10.3109/00365513.2012.679963